4.6 Article

A Novel Analysis Method for Evaluating the Interplay of Oxygen and Ionizing Radiation at the Gene Level

期刊

FRONTIERS IN GENETICS
卷 12, 期 -, 页码 -

出版社

FRONTIERS MEDIA SA
DOI: 10.3389/fgene.2021.597635

关键词

hypoxia; whole genome analysis; cluster analysis; immune response; radiation; principal component analysis

资金

  1. Deutsche Forschungsgemeinschaft [GSC129]
  2. AIRC
  3. European Union's Horizon 2020 Research and Innovation Programme under the Marie Sklodowska-Curie grant [800924]
  4. German Cancer Research Center

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This study explored the interaction between hypoxia and ionizing radiation on gene expression, revealing 37 genes consistently regulated across all cell lines studied. DNA-repair related genes were down-regulated post-irradiation, while cell cycle-dependent genes showed consistent up-regulation. The results emphasize the importance of considering both oxygen and radiation effects in analyzing gene response to cancer radiation treatment.
Whilst the impact of hypoxia and ionizing radiations on gene expression is well-understood, the interplay of these two effects is not. To better investigate this aspect at the gene level human bladder, brain, lung and prostate cancer cell lines were irradiated with photons (6 Gy, 6 MV LINAC) in hypoxic and normoxic conditions and prepared for the whole genome analysis at 72 h post-irradiation. The analysis was performed on the obtained 20,000 genes per cell line using PCA and hierarchical cluster algorithms to extract the most dominant genes altered by radiation and hypoxia. With the help of the introduced novel radiation-in-hypoxia and oxygen-impact profiles, it was possible to overcome cell line specific gene regulation patterns. Based on that, 37 genes were found to be consistently regulated over all studied cell lines. All DNA-repair related genes were down-regulated after irradiation, independently of the oxygen state. Cell cycle-dependent genes showed up-regulation consistent with an observed change in cell population in the S and G2/M phases of the cell cycle after irradiation. Genes behaving oppositely in their regulation behavior when changing the oxygen concentration and being irradiated, were immunoresponse and inflammation related genes. The novel analysis method, and by consequence, the results presented here have shown how it is important to consider the two effects together (oxygen and radiation) when analyzing gene response upon cancer radiation treatment. This approach might help to unrevel new gene patterns responsible for cancer radioresistance in patients.

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