4.5 Article

LncRNA PROX1-AS1 Facilitates Gastric Cancer Progression via miR-877-5p/PD-L1 Axis

期刊

CANCER MANAGEMENT AND RESEARCH
卷 13, 期 -, 页码 2669-2680

出版社

DOVE MEDICAL PRESS LTD
DOI: 10.2147/CMAR.S275352

关键词

PROX1-AS1; miR-877-5p; PD-L1; proliferation; apoptosis; gastric cancer

类别

资金

  1. Changshu Municipal Health and Family Planning Commission Science and Technology Plan Youth Project [cswsq201809]
  2. Suzhou science and technology development plan project (Livelihood Science and technology-Basic Research on medical and health application) [SYSD2018020]

向作者/读者索取更多资源

The study revealed that PROX1-AS1 plays a role in gastric cancer progression through the miR-877-5p/PD-L1 axis, suggesting it may serve as a new therapeutic target for the diagnosis and treatment of GC patients.
Introduction: Growing evidences imply that multiple long non-coding RNAs (lncRNAs) play a significant role in the treatment of cancer. Therefore, it is of great significance to discover new biomarkers or therapeutic targets of gastric cancer (GC). However, the potential molecular mechanism of lncPROX1-AS1 in GC remains unknown. The objective of current study is to investigate the effect of PROX1-AS1 in GC. Methods: Thus, we detect that PROX1-AS1 is over-expressed in tissues and cell lines of GC using qRT-PCR analysis. CCK-8, colony formation, flow cytometry, wounding healing and transwell analyses were performed to explore the effect of PROX1-AS1 on GC malignant behaviors. Results: It is further disclosed that silencing of PROX1-AS1 represses cell proliferation, migration, and invasion, whereas promotes cell apoptosis in GC. Bioinformatics analysis suggests that miR-877-5p is negatively regulated by PROX1-AS1 and ectopic of miR-877-5p alleviates the malignant behaviors of GC. Subsequently, miR-877-5p suppresses the activity of PD-L1-3' UTR. At last, rescue assays demonstrated that the GC progression is suppressed by sh-PROX1-AS1 and facilitated on account of miR-877-5p inhibitors and then is retrieved by sh-PD-L1. Discussion: Our findings reveal that PROX1-AS1 exerts its role via miR-877-5p/PD-L1 axis in the GC progression, suggesting that PROX1-AS1 may represent a new therapeutic target for the diagnosis and treatment of GC patients.

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