期刊
FRONTIERS IN IMMUNOLOGY
卷 12, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fimmu.2021.641360
关键词
innate immunity; SARS-COV-2; spike protein; human pulmonary collectins; surfactant protein D; angiotensin converting enzyme 2
类别
资金
- Ministry of Science and Technology (MOST) in Taiwan [MOST 107-2314-B-006 -046 -MY1-3]
- Headquarters of University Advancement at the National Cheng Kung University - Ministry of Education in Taiwan
Human SP-D plays a significant role in immune surveillance against pathogens, particularly in protecting against SARS-CoV by recognizing the virus's spike glycoprotein. Studies have demonstrated the ability of SP-D to inhibit viral infection by interacting with specific strains, highlighting its therapeutic potential in combating respiratory viruses like SARS-CoV-2.
Human SP-D is a potent innate immune molecule whose presence at pulmonary mucosal surfaces allows its role in immune surveillance against pathogens. Higher levels of serum SP-D have been reported in the patients with severe acute respiratory syndrome coronavirus (SARS-CoV). Studies have suggested the ability of human SP-D to recognise spike glycoprotein of SARS-CoV; its interaction with HCoV-229E strain leads to viral inhibition in human bronchial epithelial (16HBE) cells. Previous studies have reported that a recombinant fragment of human SP-D (rfhSP-D) composed of 8 Gly-X-Y repeats, neck and CRD region, can act against a range of viral pathogens including influenza A Virus and Respiratory Syncytial Virus in vitro, in vivo and ex vivo. In this context, this study was aimed at examining the likely protective role of rfhSP-D against SARS-CoV-2 infection. rfhSP-D showed a dose-responsive binding to S1 spike protein of SARS-CoV-2 and its receptor binding domain. Importantly, rfhSP-D inhibited interaction of S1 protein with the HEK293T cells overexpressing human angiotensin converting enzyme 2 (hACE2). The protective role of rfhSP-D against SARS-CoV-2 infection as an entry inhibitor was further validated by the use of pseudotyped lentiviral particles expressing SARS-CoV-2 S1 protein; -0.5 RLU fold reduction in viral entry was seen following treatment with rfhSP-D (10 mu g/ml). These results highlight the therapeutic potential of rfhSP-D in SARS-CoV-2 infection and merit pre-clinical studies in animal models.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据