Optimization of Glutathione Adhesion Process to Modified Graphene Surfaces

The presented work shows the results of the functionalization of the graphene surface obtained by the growth on the liquid bimetallic matrices method. We used glutathione (GSH) as a peptide model, which allowed us to optimize the procedure to obtain high process efficiency. To establish the amount of GSH attached to the graphene surface, the Folina-Ciocalteu method was used, which allows the assessment of the concentration of colored reaction products with peptide bonds without the disadvantages of most methods based on direct colored reaction of peptide bonds. Samples surface morphology, quality of graphene and chemical structure in the subsequent stages of surface modification were tested-for this purpose Raman spectroscopy, scanning electron microscopy (SEM), and Fourier-transform infrared spectroscopy (FTIR) were used.

Automated summary

This study demonstrated the functionalization of graphene surface grown on liquid bimetallic matrices, using glutathione (GSH) as a peptide model to optimize process efficiency. The Folina-Ciocalteu method was used to assess the concentration of colored reaction products with peptide bonds. Various analyses such as Raman spectroscopy, SEM, and FTIR were employed to study surface morphology, graphene quality, and chemical structure after surface modification.