期刊
GENES
卷 12, 期 5, 页码 -出版社
MDPI
DOI: 10.3390/genes12050625
关键词
KRTAP1-2; polymerase chain reaction-single strand conformation polymorphism; cashmere fiber traits; gene expression
资金
- fund for Basic Research Creative Groups of Gansu Province [18JR3RA190]
- Fuxi Young Talents Fund of Gansu Agricultural University [Gaufx02Y02]
- Gansu Provincial Finance Department Project [033-041041]
- Major Scientific and Technology Project of Qingyang City
The KRTAP1-2 gene in goats has been identified and found to have multiple sequence variants that are associated with raw cashmere fiber weight. However, these variants do not affect fiber diameter and length.
Keratin-associated proteins (KAPs) and keratins determine the physical and chemical properties of cashmere fibers as they are the main components of the fibers. It has been reported that ovine KRTAP1-2 affects clean fleece weight, greasy fleece weight and yield in sheep, but the gene has not been described in goats and its effects on fiber traits are unknown. In this study, we identify the keratin-associated protein 1-2 gene (KRTAP1-2) in the goat genome and describe its effect on cashmere fiber traits in 359 Longdong cashmere goats. Six sequence variants (named CAPHI-KRTAP1-2*A to CAPHI-KRTAP1-2*F) were revealed using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) analysis. These sequences have the highest homology with ovine KRTAP1-2 sequences. There were a 60-bp deletion, a 15-bp insertion and five single nucleotide polymorphisms (SNPs) including two non-synonymous SNPs in the coding sequence. The caprine KRTAP1-2 gene was expressed in the skin tissue, but a signal was not observed for the kidneys, liver, lungs, spleen, heart and longissimus dorsi muscle. Variation in caprine KRTAP1-2 was found to be associated with raw cashmere fiber weight, but not with fiber diameter and length.
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