期刊
GENES
卷 12, 期 3, 页码 -出版社
MDPI
DOI: 10.3390/genes12030431
关键词
rabbit; stem cells; adipose tissue; flow cytometry; digital droplet PCR; neural differentiation
资金
- Slovak Research and Development Agency [APVV-17-0124, APVV-18-0146]
- Scientific Grant Agency of the Ministry of Education, Science, Research and Sport of the Slovak Republic
- Slovak Academy of Science [VEGA 1/0160/18, VEGA 1/0049/19]
The study characterized rabbit adipose-derived mesenchymal stem cells (rAT-MSCs) using flow cytometry and PCR methods, confirming their neurogenic differentiation ability and similarity to human AT-MSCs. These cells expressed a variety of stem cell markers and neuronal markers, showing potential for diverse research and applications.
Although the rabbit is a frequently used biological model, the phenotype of rabbit adipose-derived mesenchymal stem cells (rAT-MSCs) is not well characterized. One of the reasons is the absence of specific anti-rabbit antibodies. The study aimed to characterize rAT-MSCs using flow cytometry and PCR methods, especially digital droplet PCR, which confirmed the expression of selected markers at the mRNA level. A combination of these methods validated the expression of MSCs markers (CD29, CD44, CD73, CD90 and CD105). In addition, cells were also positive for CD49f, vimentin, desmin, alpha-SMA, ALDH and also for the pluripotent markers: NANOG, OCT4 and SOX2. Moreover, the present study proved the ability of rAT-MSCs to differentiate into a neurogenic lineage based on the confirmed expression of neuronal markers ENO2 and MAP2. Obtained results suggest that rAT-MSCs have, despite the slight differences in marker expression, the similar phenotype as human AT-MSCs and possess the neurodifferentiation ability. Accordingly, rAT-MSCs should be subjected to further studies with potential application in veterinary medicine but also, in case of their cryopreservation, as a source of genetic information of endangered species stored in the gene bank.
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