期刊
GENES
卷 12, 期 4, 页码 -出版社
MDPI
DOI: 10.3390/genes12040475
关键词
telomeres; TERRA; lncRNA; sperm telomere length; ART
资金
- Italian Ministry of University and Research [2017S9KTNE]
Telomeres, particularly sperm telomere length (STL) and the long non-coding RNA TERRA, have been identified as potential biomarkers of male fertility and embryo quality. The reduced expression of TERRA in semen and shorter STL in men undergoing assisted reproductive technologies (ART) suggest a link between these factors and male infertility, highlighting the importance of TERRA in maintaining sperm telomere integrity during gametogenesis.
Telomeres are considered to be an internal biological clock, and their progressive shortening has been associated with the risk of age-related diseases and reproductive alterations. Over recent years, an increasing number of studies have focused on the association between telomere length and fertility, identifying sperm telomere length (STL) as a novel biomarker of male fertility. Although typically considered to be repeated DNA sequences, telomeres have recently been shown to also include a long non-coding RNA (lncRNA) known as TERRA (telomeric repeat-containing RNAs). Interestingly, males with idiopathic infertility show reduced testicular TERRA expression, suggesting a link between TERRA and male fertility. The aim of this study was to investigate the role of seminal TERRA expression in embryo quality. To this end, STL and TERRA expression were quantified by Real Time qPCR in the semen of 35 men who underwent assisted reproductive technologies (ART) and 30 fertile men. We found that TERRA expression in semen and STL was reduced in patients that underwent ART (both p < 0.001). Interestingly, TERRA and STL expressions were positively correlated (p = 0.010), and TERRA expression was positively associated with embryo quality (p < 0.001). These preliminary findings suggest a role for TERRA in the maintenance of sperm telomere integrity during gametogenesis, and for the first time, TERRA expression was found as a predictive factor for embryo quality in the setting of assisted reproduction.
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