期刊
FRONTIERS IN PHARMACOLOGY
卷 12, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fphar.2021.651305
关键词
β -caryophyllene; gout; monosodium urate; nucleotide-binding oligomerization domain-like receptor protein 3; nuclear factor kappa-B
资金
- Natural Science Foundation of China [81472972]
- Innovative research program for graduates of Central South University [2020zzts807]
The study explored the anti-inflammatory effects of beta-caryophyllene on MSU crystal-induced acute gouty arthritis. Beta-caryophyllene significantly reduced inflammation in the ankle joints and decreased serum cytokine levels in gouty arthritis rats. Furthermore, it inhibited the expressions of inflammatory markers in the synovial tissue.
Gouty arthritis serves as an acute reaction initiated by the deposition of monosodium urate (MSU) crystals around the joints. In this study, the anti-inflammatory effects of phytochemical beta-caryophyllene on MSU crystal-induced acute gouty arthritis in vivo and in silico were explored. Through bioinformatics methods and molecular docking, it screened the specific influence pathway of beta-caryophyllene on gout. Certain methods including enzyme-linked immunosorbent assay, western blotting, and immunohistochemical staining were adopted to quantify. beta-caryophyllene significantly reduced inflammation and function of ankle joints in MSU Crystals-induced gouty arthritis rats, while decreasing serum cytokine levels. Furthermore, it inhibited the expressions of NLRP3, Caspase-1, ASC, TLR4, MyD88, p65, and IL-1 beta in the synovial tissue so as to reduce inflammation and protect ankle joints' function. A new research approach in which beta-caryophyllene treatment to acute attacks of gout is provided through the research results.
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