期刊
CATALYSTS
卷 11, 期 5, 页码 -出版社
MDPI
DOI: 10.3390/catal11050580
关键词
Ganoderma lucidum; ganoderic acid A; triterpenoid; glycosyltransferase; cyclodextrin glucanotransferase; saponin
资金
- Ministry of Science and Technology of Taiwan
- MOST [108-2221-E-024-008-MY2]
Ganoderma lucidum is a medicinal fungus with triterpenoids as its main bioactive compounds. Through enzymatic reactions, various triterpenoid glycosides can be synthesized, which have higher aqueous solubility and potential applications in future drug development.
Ganoderma lucidum is a medicinal fungus whose numerous triterpenoids are its main bioactive constituents. Although hundreds of Ganoderma triterpenoids have been identified, Ganoderma triterpenoid glycosides, also named triterpenoid saponins, have been rarely found. Ganoderic acid A (GAA), a major Ganoderma triterpenoid, was synthetically cascaded to form GAA-15-O-beta-glucopyranoside (GAA-15-G) by glycosyltransferase (BtGT_16345) from Bacillus thuringiensis GA A07 and subsequently biotransformed into a series of GAA glucosides by cyclodextrin glucanotransferase (Toruzyme(R) 3.0 L) from Thermoanaerobacter sp. The optimal reaction conditions for the second-step biotransformation of GAA-15-G were found to be 20% of maltose; pH 5; 60 degrees C. A series of GAA glucosides (GAA-G2, GAA-G3, and GAA-G4) could be purified with preparative high-performance liquid chromatography (HPLC) and identified by mass and nucleic magnetic resonance (NMR) spectral analysis. The major product, GAA-15-O-[alpha-glucopyranosyl-(1(R)4)-beta-glucopyranoside] (GAA-G2), showed over 4554-fold higher aqueous solubility than GAA. The present study demonstrated that multiple Ganoderma triterpenoid saponins could be produced by sequential actions of BtGT_16345 and Toruzyme(R), and the synthetic strategy that we proposed might be applied to many other Ganoderma triterpenoids to produce numerous novel Ganoderma triterpenoid saponins in the future.
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