期刊
BIOLOGY OPEN
卷 10, 期 4, 页码 -出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/bio.058602
关键词
X chromosome reactivation (XCR); X chromosome inactivation (XCI); Primordial germ cell (PGC); Momiji system; Reprogramming; Imaging
类别
资金
- Ministry of Education, Culture, Sports, Science and Technology [18H04892, 19H05279, 20H03443]
- Takeda Science Foundation
- Grants-in-Aid for Scientific Research [19H05279, 18H04892, 20H03443] Funding Source: KAKEN
X chromosome reactivation (XCR) progresses along with the proliferation and clustering of primordial germ cells (PGCs) within the genital ridge, with epigenetic memory erasure timing varying according to the locus of X-linked genes.
X chromosome inactivation (XCI), determined during development, remains stable after embryonic cell divisions. However, primordial germ cells (PGCs) are exceptions in that XCI is reprogrammed and inactivated X chromosomes are reactivated. Although interactions between PGCs and somatic cells are thought to be important for PGC development, little is known about them. Here, we performed imaging of X chromosome reactivation (XCR) using the 'Momiji' mouse system, which canmonitor the X chromosome's inactive and active states using two color fluorescence reporter genes, and investigated whether interactions would affect XCR in PGCs. Based on their expression levels, we found that XCR of the Pgk1 locus began at embryonic day (E)10.5 and was almost complete by E13.5. During this period, PGCs became distributed uniformly in the genital ridge, proliferated, and formed clusters; XCR progressed accordingly. In addition, XCR of the Pgk1 locus preceded that of the Hprt locus, indicating that the timing of epigenetic memory erasure varied according to the locus of each of these X-linked genes. Our results indicate that XCR proceeds along with the proliferation of PGCs clustered within the genital ridge.
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