期刊
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
卷 28, 期 6, 页码 3553-3558出版社
ELSEVIER
DOI: 10.1016/j.sjbs.2021.03.029
关键词
Coalmine soil; Molecular approach; Streptomyces sp; Crude extract; Antimicrobial activity
类别
资金
- Deanship of Scientific Research at Princess Nourah Bint Abdulrahman University through the fast track Research Funding Program
This study collected 28 soil samples from coalmine sites in Telangana, India, isolating and identifying potential Streptomyces strains with BHPL-KSKU5 showing high antibacterial and antifungal activity. Molecular characterization revealed BHPL-KSKU5's relation to Streptomysis felleus strain, suggesting it as a potential source for therapeutic microbial agents.
In the current study, twenty-eight soil samples were collected from coalmine sites of Telangana, India. The isolates were purified and identified based on their culture characterization on oatmeal agar, glycerol asparagine agar, yeast extract-malt extract agar, inorganic salt starch agar, and starch casein agar medium. Further, the supernatant of all the isolates were tested for antimicrobial and antifungal activities. The biochemical and microscopic studies of isolated strains results indicates the potential isolate strains belongs to Streptomyces genus. Among all the strains the biological activity of BHPL-KSKU5 showed higher anti-bacterial and anti-funagal activity. The molecular characterization of BHPL-KSKU5 16s rDNA gene sequence and phylogenetic tree showed that is mostly related to the Streptomysis felleus (S. felleus) strain. This isolate was submitted to gene bank NCBI with accession number MH553077. In addition, physiological studies such as utilization of carbon, nitrogen, amino acid sources of potential isolated were studied. Further, optimization, purification and characterization of the novel compound producing strain may be helpful for discovering the new therapeutic microbial agent. (c) 2021 The Authors. Published by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据