4.6 Article

A Fluidics-Based Biosensor to Detect and Characterize Inhibition Patterns of Organophosphate to Acetylcholinesterase in Food Materials

期刊

MICROMACHINES
卷 12, 期 4, 页码 -

出版社

MDPI
DOI: 10.3390/mi12040397

关键词

electrochemical biosensors; acetylcholinesterase; organophosphate

资金

  1. NSF [1917105, 1926818]
  2. NIH R44 [OD024874]
  3. startup fund from the Henry Samueli School of Engineering, UC Irvine
  4. NSF REU Supplemental Funds [1917105]
  5. UC Irvine's UROP fellowships
  6. Direct For Social, Behav & Economic Scie [1926818] Funding Source: National Science Foundation
  7. Division Of Behavioral and Cognitive Sci [1926818] Funding Source: National Science Foundation
  8. Div Of Chem, Bioeng, Env, & Transp Sys
  9. Directorate For Engineering [1917105] Funding Source: National Science Foundation

向作者/读者索取更多资源

A chip-based electrochemical biosensor was developed for detecting organophosphate in food materials, utilizing the inhibition of acetylcholinesterase activity as the sensing principle. The sensor showed high sensitivity and stability, with significant potential for detecting organophosphates in real samples.
A chip-based electrochemical biosensor is developed herein for the detection of organophosphate (OP) in food materials. The principle of the sensing platform is based on the inhibition of dimethoate (DMT), a typical OP that specifically inhibits acetylcholinesterase (AChE) activity. Carbon nanotube-modified gold electrodes functionalized with polydiallyldimethylammonium chloride (PDDA) and oxidized nanocellulose (NC) were investigated for the sensing of OP, yielding high sensitivity. Compared with noncovalent adsorption and deposition in bovine serum albumin, bioconjugation with lysine side chain activation allowed the enzyme to be stable over three weeks at room temperature. The total amount of AChE was quantified, whose activity inhibition was highly linear with respect to DMT concentration. Increased incubation times and/or DMT concentration decreased current flow. The composite electrode showed a sensitivity 4.8-times higher than that of the bare gold electrode. The biosensor was challenged with organophosphate-spiked food samples and showed a limit of detection (LOD) of DMT at 4.1 nM, with a limit of quantification (LOQ) at 12.6 nM, in the linear range of 10 nM to 1000 nM. Such performance infers significant potential for the use of this system in the detection of organophosphates in real samples.

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