期刊
LIGHT-SCIENCE & APPLICATIONS
卷 10, 期 1, 页码 -出版社
SPRINGERNATURE
DOI: 10.1038/s41377-021-00513-w
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资金
- National Key Research and Development Program of China [2017YFC0110100]
- National Natural Science Foundation of China [61805272]
HiFi-SIM is a high-fidelity SIM reconstruction algorithm that can reduce artifacts and improve axial sectioning without loss of fine structures, making it a daily imaging tool with lower calibration and adjustment requirements.
Structured illumination microscopy (SIM) has become a widely used tool for insight into biomedical challenges due to its rapid, long-term, and super-resolution (SR) imaging. However, artifacts that often appear in SIM images have long brought into question its fidelity, and might cause misinterpretation of biological structures. We present HiFi-SIM, a high-fidelity SIM reconstruction algorithm, by engineering the effective point spread function (PSF) into an ideal form. HiFi-SIM can effectively reduce commonly seen artifacts without loss of fine structures and improve the axial sectioning for samples with strong background. In particular, HiFi-SIM is not sensitive to the commonly used PSF and reconstruction parameters; hence, it lowers the requirements for dedicated PSF calibration and complicated parameter adjustment, thus promoting SIM as a daily imaging tool.
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