Characterization of BoaCRTISO Reveals Its Role in Carotenoid Biosynthesis in Chinese Kale

Carotenoids are organic pigments that play an important role in both plant coloration and human health; they are a critical subject in molecular breeding due to growing demand for natural molecules in both food and medicine. In this study, we focus upon characterizing BoaCRTISO, the carotenoid isomerase gene before the branch of the carotenoid biosynthetic pathway, which is expressed in all organs and developmental stages of Chinese kale, and BoaCRTISO, which is located in the chloroplast. The expression of BoaCRTISO is induced by strong light, red and blue combined light, and gibberellic acid treatment, but it is suppressed by darkness and abscisic acid treatment. We obtained BoaCRTISO-silenced plants via virus-induced gene silencing technology, and the silence efficiencies ranged from 52 to 77%. The expressions of most carotenoid and chlorophyll biosynthetic genes in BoaCRTISO-silenced plants were downregulated, and the contents of carotenoids and chlorophyll were reduced. Meanwhile, BoaCRTISO-silenced plants exhibited phenotypes of yellowing leaves and inhibited growth. This functional characterization of BoaCRTISO provides insight for the biosynthesis and regulation of carotenoid in Chinese kale.

Automated summary

Carotenoids are organic pigments that are important for plant coloration and human health. The study focused on characterizing BoaCRTISO, a carotenoid isomerase gene in Chinese kale, and found that silencing this gene led to downregulation of carotenoid and chlorophyll biosynthetic genes, as well as reduced contents of carotenoids and chlorophyll, resulting in yellowing leaves and inhibited growth in plants. This provides insight into the biosynthesis and regulation of carotenoids in Chinese kale.