Three Ribosomal Operons of Escherichia coli Contain Genes Encoding Small RNAs That Interact With Hfq and CsrA in vitro

Three out of the seven ribosomal RNA operons in Escherichia coli end in dual terminator structures. Between the two terminators of each operon is a short sequence that we report here to be an sRNA gene, transcribed as part of the ribosomal RNA primary transcript by read-through of the first terminator. The sRNA genes (rrA, rrB and rrF) from the three operons (rrnA, rrnB and rrnD) are more than 98% identical, and pull-down experiments show that their transcripts interact with Hfq and CsrA. Deletion of rrA, B, F, as well as overexpression of rrB, only modestly affect known CsrA-regulated phenotypes like biofilm formation, pgaA translation and glgC translation, and the role of the sRNAs in vivo may not yet be fully understood. Since RrA, B, F are short-lived and transcribed along with the ribosomal RNA components, their concentration reflect growth-rate regulation at the ribosomal RNA promoters and they could function to fine-tune other growth-phase-dependent processes in the cell. The primary and secondary structure of these small RNAs are conserved among species belonging to different genera of Enterobacteriales.

Automated summary

Three out of seven ribosomal RNA operons in Escherichia coli end in dual terminator structures, with sRNA genes (rrA, rrB, rrF) between the terminators showing high sequence similarity and interacting with Hfq and CsrA. Deletion or overexpression of these sRNAs has modest effects on known CsrA-regulated phenotypes, indicating their potential role in fine-tuning growth-phase-dependent processes in the cell. The conserved primary and secondary structure of these small RNAs across different genera of Enterobacteriales suggests a common functional significance.