4.6 Article

Identification of Novel Sensitive and Reliable Serovar-Specific Targets for PCR Detection of Salmonella Serovars Hadar and Albany by Pan-Genome Analysis

期刊

FRONTIERS IN MICROBIOLOGY
卷 12, 期 -, 页码 -

出版社

FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2021.605984

关键词

Salmonella; C2 serogroups; serovar-specific molecular targets; PCR; pan-genome analysis

资金

  1. National Key Research and Development Program of China [2017YFC1600403]
  2. Local Innovative and Research Teams Project of Guangdong PEARL River Talents Program [2017BT01S174]
  3. Natural Science Foundation of Guangdong Province, China [2018A030313442]
  4. GDAS' Project of Science and Technology Development [2018GDASCX-0401]

向作者/读者索取更多资源

The accurate and rapid classification of Salmonella serovars is crucial for the identification of isolates involved in disease in humans and animals. This study successfully identified specific genes for Albany and Hadar serovars in Salmonella C2 serogroups and designed PCR primers with high specificity. The sensitivity and detection limits of the specific PCR method in food samples were also determined, highlighting the potential for rapid and sensitive detection of these specific serovars.
The accurate and rapid classification of Salmonella serovars is an essential focus for the identification of isolates involved in disease in humans and animals. The purpose of current research was to identify novel sensitive and reliable serovar-specific targets and to develop PCR method for Salmonella C2 serogroups (O:8 epitopes) in food samples to facilitate timely treatment. A total of 575 genomic sequences of 16 target serovars belonging to serogroup C2 and 150 genomic sequences of non-target serovars were analysed by pan-genome analysis. As a result, four and three specific genes were found for serovars Albany and Hadar, respectively. Primer sets for PCR targeting these serovar-specific genes were designed and evaluated based on their specificity; the results showed high specificity (100%). The sensitivity of the specific PCR was 2.8 x 10(1)-10(3) CFU/mL and 2.3 x 10(3)-10(4) CFU/mL for serovars Albany and Hadar, respectively, and the detection limits were 1.04 x 10(3)-10(4) CFU/g and 1.16 x 10(4)-10(5) CFU/g in artificially contaminated raw pork samples. Furthermore, the potential functions of these serovar-specific genes were analysed; all of the genes were functionally unknown, except for one specific serovar Albany gene known to be a encoded secreted protein and one specific gene for serovars Hadar and Albany that is a encoded membrane protein. Thus, these findings demonstrate that pan-genome analysis is a precious method for mining new high-quality serovar-targets for PCR assays or other molecular methods that are highly sensitive and can be used for rapid detection of Salmonella serovars.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.6
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据