期刊
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
卷 54, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.algal.2020.102172
关键词
Myrmecia incisa; Molecular characterization; Immunoelectron microscopy; GFP fusion; Enzyme activity
资金
- National Natural Science Foundation of China (NSFC) [31772821]
- Science and Technology Commission of Shanghai Municipality [20ZR1423900]
- NSFC-Shandong Joint Fund [U1706209]
- State Double First-Class Discipline Project of Aquaculture
Microalgae are a rich source of triacylglycerol (TAG), with the newly identified MiGPAT2 in Myrmecia incisa playing a key role in de novo TAG biosynthesis. MiGPAT1 and MiGPAT2 show differences in subcellular localization and function, with MiGPAT2 predominantly involved in TAG biosynthesis and MiGPAT1 contributing to TAG production indirectly through phospholipid formation.
Microalgae represent a rich and naturally occurring source of triacylglycerol (TAG), and the microalgae-derived TAG has enormous potential in bioenergy, bio-based materials and other agricultural innovations. Glycerol-3-phosphate acyltransferase (GPAT) is a key enzyme that catalyzes the biosynthesis of TAG. Previously our lab has reported the first GPAT in the oleaginous alga Myrmecia incisa, and in the present study, a new GPAT was further identified from the same alga, which was designated as MiGPAT2. A full-length cDNA of MiGPAT2 consisting of a 1374-bp ORF, a 153-bp 5'-UTR and a 271-bp 3' -UTR was cloned. The putative protein composed of 457 amino acids, possessing the HX4D motif that acts as the activity center of GPAT catalysis. Emphasis was put on the comparison between two MiGPATs regarding subcellular localization and TAG synthesis function. A polyclonal and monoclonal antibody were separately prepared for MiGPAT1 and MiGPAT2, and the following immunogold labeling showed they were localized on chloroplast and endoplasmic reticulum (ER), respectively. This was further confirmed by the GFP fusion studies. Both MiGPATs were expressed heterologously in a GPATdeficient yeast mutant, and it was found that MiGPAT2 played key roles in the de novo TAG biosynthesis, whereas MiGPAT1 was more involved in the phospholipid formation, contributing to TAG production via an indirect way. Findings of the present study expanded our knowledge on functional and evolutionary relationships of GPAT members in M. incisa, suggesting the possibility of genetic manipulation of GPAT in this alga for enhanced TAG production.
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