Microbial Detoxification of Dimethoate and Methomyl Residues in Aqueous Media

The extensive and random application of major organic pollutants, mainly pesticides, threatens ecosystems and human health. The present study was conducted to isolate and identify microorganisms from some water resources contaminated with pesticides. We investigated the ability of the identified microbes to grow in water spiked with dimethoate and methomyl. We also evaluated the potential effect of the identified microbial isolates on dimethoate and methomyl biodegradation in water. In addition, the total detoxification of dimethoate and methomyl residues in water after treatment with the most effective microbial isolates was confirmed using toxicity tests and analyzing biochemical parameters and histopathological changes in the kidney and liver of treated rats. The microbial isolates were identified as Xanthomonas campestris pv. Translucens and Aspergillus fumigates. Results showed that X. campestris pv. Translucens and A. fumigatus grow in media supplemented with dimethoate and methomyl faster than in other media without both pesticides. About 97.8% and 91.2% of dimethoate and 95% and 87.8% of methomyl (initial concentration of both 5 mg L-1) were biodegraded within 32 days of incubation with X. campestris pv. Translucens and A. fumigatus, respectively. There was no remaining toxicity in rats treated with dimethoate- and methomyl-contaminated water with respect to biochemical parameters and histopathological changes. Collectively, the identified bacterial isolate showed high potential for the complete degradation of dimethoate and methomyl residues in water.

Automated summary

The study isolated Xanthomonas campestris pv. Translucens and Aspergillus fumigatus as microbial isolates capable of degrading dimethoate and methomyl residues in water. These isolates showed fast growth and degradation rates in water spiked with pesticides, with up to 97.8% and 91.2% of dimethoate, and 95% and 87.8% of methomyl being biodegraded within 32 days. Toxicity tests on rats confirmed the absence of remaining toxicity after treatment with these microbial isolates, indicating their high potential for complete degradation of pesticide residues.