期刊
POLYMERS
卷 13, 期 6, 页码 -出版社
MDPI
DOI: 10.3390/polym13060885
关键词
thermosensitive hydrogels; MIN6 cells; subcutaneous transplantation; magnetic resonance imaging; bioluminescence imaging
资金
- Chang Gung Memorial Hospital, Taiwan [CMRPG3F0711-3, CMRPG3H0261, CMRPG3G1961]
- Chang Gung Memorial Hospital
- National Tsing Hua University Joint Research Program CGMH-NTHU 2019, Taiwan [CORPG3I0031, 108Q2505E1]
- National Tsing Hua University Joint Research Program CGMH-NTHU 2020, Taiwan [CORPG3K0031, 109Q2513E1]
The study demonstrated the feasibility of using mPEG-poly(Ala) hydrogels as a scaffold for beta-cell encapsulation and transplantation. Furthermore, MR and bioluminescence imaging proved to be useful noninvasive tools for detecting and monitoring the embedded MIN6 cells at a subcutaneous site.
Recently, we demonstrated the feasibility of subcutaneous transplantation of MIN6 cells embedded in a scaffold with poly(ethylene glycol) methyl ether (mPEG)-poly(Ala) hydrogels. In this study, we further tracked these grafts using magnetic resonance (MR) and bioluminescence imaging. After being incubated overnight with chitosan-coated superparamagnetic iron oxide (CSPIO) nanoparticles and then mixed with mPEG-poly(Ala) hydrogels, MIN6 cells appeared as dark spots on MR scans. For in vivo experiments, we transfected MIN6 cells with luciferase and/or incubated them overnight with CSPIO overnight; 5 x 10(6) MIN6 cells embedded in mPEG-poly(Ala) hydrogels were transplanted into the subcutaneous space of each nude mouse. The graft of CSPIO-labeled MIN6 cells was visualized as a distinct hypointense area on MR images located at the implantation site before day 21. However, this area became hyperintense on MR scans for up to 64 days. In addition, positive bioluminescence images were also observed for up to 64 days after transplantation. The histology of removed grafts showed positive insulin and iron staining. These results indicate mPEG-poly(Ala) is a suitable scaffold for beta-cell encapsulation and transplantation. Moreover, MR and bioluminescence imaging are useful noninvasive tools for detecting and monitoring mPEG-poly(Ala) hydrogel-embedded MIN6 cells at a subcutaneous site.
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