期刊
OPEN BIOLOGY
卷 11, 期 4, 页码 -出版社
ROYAL SOC
DOI: 10.1098/rsob.200395
关键词
diatoms; nitrogen; transporters; metagenomics; gene phylogeny
资金
- Green and Circular Economy (GECE) - Italian Ministry of University Education and Research (MIUR) [856]
- Open University-SZN PhD program
Diatoms are a diverse group of phytoplankton with chimeric genomes containing genes from bacteria, animals, and plants. A comprehensive characterization of the Nitrate Transporter 1/Peptide Transporter Family (NPF) in diatoms revealed complex structures and functions that may play a crucial role in diatoms' adaptability to different environmental conditions.
Diatoms are one of the major and most diverse groups of phytoplankton, with chimeric genomes harbouring a combination of genes of bacterial, animal and plant origin. They have developed sophisticated mechanisms to face environmental variations. In marine environments, nutrients concentration shows significant temporal and spatial variability, influencing phytoplankton growth. Among nutrients, nitrogen, present at micromolar levels, is often a limiting resource. Here, we report a comprehensive characterization of the Nitrate Transporter 1/Peptide Transporter Family (NPF) in diatoms, diNPFs. NPFs are well characterized in many organisms where they recognize a broad range of substrates, ranging from short-chained di- and tri-peptides in bacteria, fungi and mammals to a wide variety of molecules including nitrate in higher plants. Scarce information is available for diNPFs. We integrated-omics, phylogenetic, structural and expression analyses, to infer information on their role in diatoms. diNPF genes diverged to produce two distinct clades with strong sequence and structural homology with either bacterial or plant NPFs, with different predicted sub-cellular localization, suggesting that the divergence resulted in functional diversification. Moreover, transcription analysis of diNPF genes under different laboratory and environmental growth conditions suggests that diNPF diversification led to genetic adaptations that might contribute to diatoms ability to flourish in diverse environmental conditions.
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