期刊
JOURNAL OF GINSENG RESEARCH
卷 45, 期 6, 页码 726-733出版社
KOREAN SOC GINSENG
DOI: 10.1016/j.jgr.2021.04.001
关键词
Panax notoginseng; Metabolites; Spatial distribution; Steaming process; MALDI-MS imaging
资金
- Taishan Scholars Program of Shandong Province, China
- key project at the central government level: The Ability Establishment of Sustainable Use for Valuable Chinese Medicine Resources, China [2060302]
- National Natural Science Foundation of China, China [21904080]
- Science, Education and Industry Integration Innovation Pilot Project (International Cooperation Project) from Qilu University of Technology (Shandong Academy of Sciences), China [2020KJC-GH08]
- Major Innovation Project of Shandong Biotechnology Technology Innovation Center, China [2019JSWGCCXZX001]
A highly sensitive MALDI-MS imaging method was developed to visualize the spatial distributions and spatiotemporal changes of metabolites in different botanical parts of Panax notoginseng. Metabolites that changed during the steaming of Panax notoginseng were successfully screened and imaged, enhancing our understanding of its metabolic networks.
Background: Panax notoginseng is a highly valued medicinal herb used widely in China and many Asian countries. Its root and rhizome have long been used for the treatment of cardiovascular and hematological diseases. Imaging the spatial distributions and dynamics of metabolites in heterogeneous plant tissues is significant for characterizing the metabolic networks of Panax notoginseng, and this will also provide a highly informative approach to understand the complex molecular changes in the processing of Panax notoginseng. Methods: Here, a high-sensitive MALDI-MS imaging method was developed and adopted to visualize the spatial distributions and spatiotemporal changes of metabolites in different botanical parts of Panax notoginseng. Results: A wide spectrum of metabolites including notoginsenosides, ginsenosides, amino acids, dencichine, gluconic acid, and low-molecular-weight organic acids were imaged in Panax notoginseng rhizome and root tissues for the first time. Moreover, the spatiotemporal alterations of metabolites during the steaming of Panax notoginseng root were also characterized in this study. And, a series of metabolites such as dencichine, arginine and glutamine that changed with the steaming of Panax notoginseng were successfully screened out and imaged. Conclusion: These spatially-resolved metabolite data not only enhance our understanding of the Panax notoginseng metabolic networks, but also provide direct evidence that a serious of metabolic alterations occurred during the steaming of Panax notoginseng. (C) 2021 The Korean Society of Ginseng. Publishing services by Elsevier B.V.
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