The First Polish Isolate of a Novel Species Pectobacterium aquaticum Originates from a Pomeranian Lake

Pectinolytic bacteria from the genus Pectobacterium cause high economic losses in various crops, vegetables, and ornamentals including potato. Thus far, these strains have been isolated from distinct environments such as rotten or asymptomatic plants, soil, and waterways. The prevalence of soft rot Pectobacteriaceae in different depths of Pomeranian lakes was performed by a qualified scuba diver over 2 years of monitoring. It allowed for the isolation and broad characterization of a strain from the newly established species Pectobacterium aquaticum. Phylogenetic analysis on the sequences of dnaX and recA genes revealed the highest similarity of this strain to P. aquaticum CFBP 8637(T). In addition to the determination of analytical profile index (API 20E), we discovered that this strain possesses a smooth form of a lipopolysaccharide with O-polysaccharide consisting of mannose, glucose, and abequose. Moreover, the characterized strain, described as P. aquaticum IFB5637, produced plant-cell-wall-degrading enzymes, such as pectinases, cellulases, proteases, and was capable of macerating potato and chicory tissues under laboratory conditions. In view of more frequent irrigation of seed potato fields resulting from the ongoing climate warming, it is important to monitor the occurrence of potential disease-causing agents in natural waterways.

Automated summary

Pectinolytic bacteria from the genus Pectobacterium have been found to cause economic losses in various crops and plants, including potato. A new strain of Pectobacterium aquaticum was isolated and characterized from Pomeranian lakes, showing the ability to degrade plant cell walls and macerate tissues. Monitoring for potential disease-causing agents in natural waterways is essential due to increased irrigation in seed potato fields caused by climate warming.