期刊
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
卷 18, 期 10, 页码 -出版社
MDPI
DOI: 10.3390/ijerph18105345
关键词
urban particulate matter; children; early biological effects; mucosa buccal cells; micronuclei test; comet assay; polycyclic aromatic hydrocarbons; metals; in vitro mutagenicity; Ames test
资金
- Research Centre Q-TECH Research and Study Centre (Quality and Technology Assessment, Governance and Communication Strategies in Health Systems), University of Brescia
- Lombardy Regional Authority
The study found that PAHs in particulate matter may be associated with micronuclei formation in children's buccal mucosa cells, different sized fractions of particulate matter have varying effects on DNA damage and mutagenicity, and metal concentrations do not show significant correlation with mutagenic activity of particulate matter.
The aim of this paper was to investigate the relationship between micronuclei and DNA damage in children's buccal mucosa cells and the genotoxicity and mutagenicity of the different sized fractions of particulate matter as well as the concentration of PAHs (polycyclic aromatic hydrocarbons) and metals in particulate matter. Air particulate matter was collected by high volume samplers located near the schools attended by the children on the same days of biological samplings. The mutagenic activity was assessed in different cells in in vitro tests (Ames test on bacteria and comet test on leukocytes). Our study showed weak positive correlations between (a) the mutagenicity of the PM0.5 fraction and PAHs and (b) the micronuclei test of children's buccal cells and PAHs detected in PM0.5 and PM0.5-3 fractions. A positive correlation was also found between in vitro comet test on leukocytes and PAHs in the PM3-10 fraction. No correlation was observed for metal concentrations in each PM fraction.
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