期刊
BRAZILIAN JOURNAL OF CHEMICAL ENGINEERING
卷 32, 期 1, 页码 35-42出版社
SPRINGER HEIDELBERG
DOI: 10.1590/0104-6632.20150321s00003298
关键词
Bacillus sp.; CMCase; Sugarcane; Enzyme production; Thermophilic cellulase
资金
- Brazilian Council for Research (CNPq)
- Coordination for Higher Education Personnel Development (CAPES)
The production and characterization of cellulase from thermophilic strain Bacillus sp. C1AC5507 was studied. For enzyme production, sugarcane bagasse was used as carbon source. The produced carboxymethyl cellulase (CMCase) had a molecular weight around 55 kDa and its activity varied between 0.14 and 0.37 IU mL(-1) in conditions predicted by Response Surface Methodology. The optimum temperature and pH for the CMCase production were 70 degrees C and 7.0, respectively. The enzyme activity was inhibited mostly by Cu+2 and activated mostly by Co+2, Mn2+, Ca+2 and Fe+3. Our findings provide a contribution to the use of natural wastes such as sugarcane bagasse as substrate for growth and production of thermophilic CMCase. Further optimization to increase the production of cellulase enables the use in industrial applications.
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