期刊
BRAZILIAN JOURNAL OF CHEMICAL ENGINEERING
卷 32, 期 2, 页码 335-345出版社
BRAZILIAN SOC CHEMICAL ENG
DOI: 10.1590/0104-6632.20150322s00003304
关键词
Nitrogen limitation; Lipid productivity; Multistage; Microalgae; Biodiesel
资金
- Mexican Council of Science and Technology (CONACyT) [145521]
- Secretariat of Public Education of Mexico (SEP) project [PROMEP 103.5/09/7341]
Chlorella vulgaris and Scenedesmus obliquus were grown in artificial-wastewater using a new nitrogen-limitation strategy aimed at increasing lipid productivity. This strategy consisted in a multi-stage process with sequential reduction of N-NH4 concentration (from 90 to 60, 40, and 20 mg.L-1) to promote a balance between cell growth and lipid accumulation. Lipid productivity was compared against a reference process consisting of nitrogen reduction in two stages, where the nitrogen concentration was suddenly reduced from 90 mg.L-1 to three different concentrations (10, 20, and 30 mg.L-1). In the multi-stage mode, only C. vulgaris exhibited a net lipid-productivity increase. Lipid content of S. obliquus did not present a significant increase, thus decreasing lipid productivity. The highest lipid productivities were observed in the two-stage mode for both S. obliquus and C. vulgaris (194.9 and 133.5 mg.L-1.d(-1), respectively), and these values are among the highest reported in the literature to date.
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