期刊
ADVANCED HEALTHCARE MATERIALS
卷 10, 期 10, 页码 -出版社
WILEY
DOI: 10.1002/adhm.202100266
关键词
all‐ aqueous fabrication; aqueous two‐ phase systems; cell‐ laden capsules; interfacial complexation
资金
- European Research Council [ERC-2014-ADG-669858]
- FCT/MEC [UIDB/50011/2020, UIDP/50011/2020]
- FEDER under the PT2020 Partnership Agreement
- Programa Operacional Competitividade e InternacionalizacAo, in the component FEDER
- national funds (OE) through FCT/MCTES [PTDC/BTM-ORG/30770/2017]
- [CEECIND/03605/2017]
- Fundação para a Ciência e a Tecnologia [PTDC/BTM-ORG/30770/2017] Funding Source: FCT
The study describes the one-step fast formation of robust polymeric capsules through interfacial complexation of oppositely charged polyelectrolytes in an all-aqueous environment, which is compatible with the encapsulation of mesenchymal stem/stromal cells (MSCs) and microcarriers. Cell aggregation and secretion over time can be tailored by providing cells with static or dynamic (bioreactor) environments.
The therapeutic effectiveness and biological relevance of technologies based on adherent cells depend on platforms that enable long-term culture in controlled environments. Liquid-core capsules have been suggested as semipermeable moieties with spatial homogeneity due to the high mobility of all components in their core. The lack of cell-adhesive sites in liquid-core structures often hampers their use as platforms for stem cell-based technologies for long-term survival and cell-directed self-organization. Here, the one-step fast formation of robust polymeric capsules formed by interfacial complexation of oppositely charged polyelectrolytes in an all-aqueous environment, compatible with the simultaneous encapsulation of mesenchymal stem/stromal cells (MSCs) and microcarriers, is described. The adhesion of umbilical cord MSCs to polymeric microcarriers enables their aggregation and culture for more than 21 days in capsules prepared either manually by dropwise addition, or by scalable electrohydrodynamic atomization, generating robust and stable capsules. Cell aggregation and secretion overtime can be tailored by providing cells with static or dynamic (bioreactor) environments.
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