期刊
ACS SYNTHETIC BIOLOGY
卷 10, 期 6, 页码 1292-1299出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssynbio.1c00036
关键词
Smarticles; antimicrobial resistance; carbapenem-resistant Enterobacterales; antimicrobial susceptibility testing; synthetic biology; bacteriophage
资金
- Roche Molecular Systems (Pleasanton, United States)
Bacterial transduction particles play a crucial role in molecular biology, and are currently attracting interest in the diagnostic and therapeutic fields. Developing effective transduction reagents remains a challenge, but the use of a synthetic biology platform shows promise for future rapid diagnostic assays.
Bacterial transduction particles were critical to early advances in molecular biology and are currently experiencing a resurgence in interest within the diagnostic and therapeutic fields. The difficulty of developing a robust and specific transduction reagent capable of delivering a genetic payload to the diversity of strains constituting a given bacterial species or genus is a major impediment to their expanded utility as commercial products. While recent advances in engineering the reactivity of these reagents have made them more attractive for product development, considerable improvements are still needed. Here, we demonstrate a synthetic biology platform derived from bacteriophage P1 as a chassis to target transduction reagents against four clinically prevalent species within the Enterobacterales order. Bacteriophage P1 requires only a single receptor binding protein to enable attachment and injection into a target bacterium. By engineering and screening particles displaying a diverse array of chimeric receptor binding proteins, we generated a potential transduction reagent for a future rapid phenotypic carbapenem-resistant Enterobacterales diagnostic assay.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据