期刊
THORACIC CANCER
卷 12, 期 13, 页码 2013-2023出版社
WILEY
DOI: 10.1111/1759-7714.14004
关键词
EMT; KIN17; Lum-A breast cancer; metastasis; migration
资金
- Guangdong Planning Project of Science and Technology [2016A020215118]
- Pearl River S&T Nova Program of Guangzhou [201710010015]
- Start-up Fund for High-level Talents of Guangdong Medical University [51301Z20200007]
- Zhongshan Social Welfare Project of Science and Technology [2019B1092]
The study found that KIN17 expression was associated with poor overall survival in breast cancer patients, especially in Luminal-A type. Knockdown of KIN17 inhibited migration and invasion of breast cancer cells, while overexpression of KIN17 promoted cell migration and invasion.
Background: Breast cancer (BC), the most common cause of cancer death in women, overtook lung cancer as the leading cause of cancer worldwide in 2020. Although many studies have proposed KIN17 as a biomarker of tumorigenesis in different cancer types, its role in tumor metastasis, particularly in BC metastasis, has been underexplored. This study aimed to explore the role of KIN17 in BC metastasis. Methods: Survival analyses was performed to identify the association between KIN17 expression and BC patient survival in silico. Using lentivirus constructs, we developed bidirectional KIN17 expression (KD, knockdown; OE, overexpression) cellular models of luminal-A (Lum-A) breast cancer MCF-7 cells. We performed in vitro wound healing, transwell with and without Matrigel assays, and in vivo tail-vein metastasis assay to evaluate the migration and invasion abilities of MCF-7 with stable KIN17 knockdown or overexpression. Western blotting was performed to compare the changes in protein expression. Results: We found that KIN17 expression was associated with poor overall survival (OS), relapse-free survival (RFS), distant metastasis-free survival (DMFS) and post-progression survival (PPS), particularly in Lum-A breast cancer patients. Later, we found that KIN17 knockdown inhibited migration and invasion of MCF-7 cells via regulating EMT-associated signaling pathways in vitro and decreases metastatic spread of the disease in vivo. In contrast, KIN17 overexpression promoted migration and invasion of MCF-7 cells in vitro and increased the metastatic spread of the disease in vivo. Conclusions: Overall, our findings provide preliminary data which suggests KIN17 of importance to target in metastatic Lum-A patients.
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