4.7 Article

Determination of a microRNA signature of protective kidney ischemic preconditioning originating from proximal tubules

期刊

SCIENTIFIC REPORTS
卷 11, 期 1, 页码 -

出版社

NATURE RESEARCH
DOI: 10.1038/s41598-021-89195-3

关键词

-

资金

  1. Medical Research Council [MC_PC_13060] Funding Source: Medline

向作者/读者索取更多资源

Ischemic preconditioning (IPC) provides optimal protection against subsequent ischemic acute kidney injury through short, repetitive sequences of preconditioning insult. Molecular analysis identified a unique profile of microRNA changes related to ischemia-reperfusion injury (IRI), predominantly localized in glomeruli and proximal tubules in the rat kidney. Pulsatile IPC reduces kidney damage post-IRI and attenuates the microRNA signal, highlighting potential candidate microRNAs as biomarkers and therapeutic targets.
Ischemic preconditioning (IPC) is effective in limiting subsequent ischemic acute kidney injury in experimental models. MicroRNAs are an important class of post-transcriptional regulator and show promise as biomarkers of kidney injury. We evaluated the time- and dose-dependence of benefit from IPC in a rat model of functional (bilateral) ischemia-reperfusion injury (IRI). We found optimal protection from subsequent injury following short, repetitive sequences of preconditioning insult. We subsequently used hybridization array and microRNA sequencing to characterize microRNA signatures of protective IPC and of IRI. These approaches identified a profile of microRNA changes consequent on IRI, that were limited by prior IPC. To localize these signals within the kidney, we used laser capture microdissection and RT-qPCR to measure microRNA abundance in nephron segments, pinpointing microRNA changes principally to glomeruli and proximal tubules. Our data describe a unique microRNA signature for IRI in the rat kidney. Pulsatile IPC reduces kidney damage following IRI and diminishes this microRNA signal. We have also identified candidate microRNAs that may act as biomarkers of injury and therapeutic targets in this context.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据