The PsbS protein and low pH are necessary and sufficient to induce quenching in the light-harvesting complex of plants LHCII

Photosynthesis is tightly regulated in order to withstand dynamic light environments. Under high light intensities, a mechanism known as non-photochemical quenching (NPQ) dissipates excess excitation energy, protecting the photosynthetic machinery from damage. An obstacle that lies in the way of understanding the molecular mechanism of NPQ is the large gap between in vitro and in vivo studies. On the one hand, the complexity of the photosynthetic membrane makes it challenging to obtain molecular information from in vivo experiments. On the other hand, a suitable in vitro system for the study of quenching is not available. Here we have developed a minimal NPQ system using proteoliposomes. With this, we demonstrate that the combination of low pH and PsbS is both necessary and sufficient to induce quenching in LHCII, the main antenna complex of plants. This proteoliposome system can be further exploited to gain more insight into how PsbS and other factors (e.g. zeaxanthin) influence the quenching mechanism observed in LHCII.

Automated summary

Photosynthesis is regulated to withstand dynamic light environments, with NPQ dissipating excess excitation energy under high light intensities. There is a gap between in vitro and in vivo studies on the molecular mechanism of NPQ, and the development of a proteoliposome system provides insights into the influence of factors like PsbS on the quenching mechanism in LHCII.