4.7 Article

The tumor suppressor archipelago E3 ligase is required for spermatid differentiation in Drosophila testis

期刊

SCIENTIFIC REPORTS
卷 11, 期 1, 页码 -

出版社

NATURE RESEARCH
DOI: 10.1038/s41598-021-87656-3

关键词

-

资金

  1. University of Szeged [5130]
  2. [NKFIH_OTKA 132155]
  3. [GINOP 2.3.2-15-2016-00032]
  4. [GINOP-2.3.2-15-2016-00035]
  5. [EFOP-3.6.1-16-2016-00008]

向作者/读者索取更多资源

In this study, researchers discovered a previously unknown tissue-specific role of the Drosophila archipelago (ago) gene in spermatid differentiation, affecting spermatid development and mitochondrial morphology. Silencing ago gene confirmed spermatogenesis defects. Mutants of ago gene in Drosophila exhibit abnormalities in spermatids, including cyst morphology, mitochondrial structures, and individualization defects.
The human orthologue of the tumor suppressor protein FBW7 is encoded by the Drosophila archipelago (ago) gene. Ago is an F-box protein that gives substrate specificity to its SCF ubiquitin ligase complex. It has a central role in multiple biological processes in a tissue-specific manner such as cell proliferation, cellular differentiation, hypoxia-induced gene expression. Here we present a previously unknown tissue-specific role of Ago in spermatid differentiation. We identified a classical mutant of ago which is semi-lethal and male-sterile. During the characterization of ago function in testis, we found that ago plays role in spermatid development, following meiosis. We confirmed spermatogenesis defects by silencing ago by RNAi in testes. The ago mutants show multiple abnormalities in elongating and elongated spermatids, including aberration of the cyst morphology, malformed mitochondrial structures, and individualization defects. Additionally, we determined the subcellular localization of Ago protein with mCherry-Ago transgene in spermatids. Our findings highlight the potential roles of Ago in different cellular processes of spermatogenesis, like spermatid individualization, and regulation of mitochondrial morphology.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据