Impact of Sinorhizobium meliloti Exopolysaccharide on Adsorption and Aggregation in the Copper(II) Ions/Supporting Electrolyte/Kaolinite System

To obtain insight into physicochemical interactions between Cu(II) ions, kaolinite, and exopolysaccharide (EPS) synthesized by Sinorhizobium meliloti Rm 1021 soil bacteria, an adsorption, electrokinetic, and aggregation study was performed in the selected systems. The obtained data showed that supporting electrolyte type affects both EPS and Cu(II) ions adsorption. For initial Cu(II) concentration 100 mg/L, 4.36 +/- 0.25 mg/g (21.80 +/- 1.00%) of the ions were adsorbed in 0.001 M NaCl and 3.76 +/- 0.20 mg/g (18.80 +/- 1.00%) in 0.001 M CaCl2. The experimental data were best fitted to the Langmuir model as well as pseudo second-order equation. The EPS adsorbed amount on kaolinite was higher in the CaCl2 electrolyte than in NaCl one. For an initial polymer concentration of 100 mg/L, the EPS adsorbed amount was 4.69 +/- 0.08 mg/g (23.45 +/- 0.40%) in 0.001 M NaCl and 5.26 +/- 0.15 mg/g (26.32 +/- 0.75%) in 0.001 M CaCl2. In the mixed system, regardless of electrolyte type, exopolysaccharide contributed to immobilization of higher amount of copper(II) ions on the clay mineral. Also, in the samples containing heavy metal ions and exopolysaccharide simultaneously, the aggregation of kaolinite particles was the strongest. The results presented in the paper may be very helpful in soil bioremediation, especially in the development of technologies reducing the mobility of heavy metals in the environment.

Automated summary

The study found that the type of supporting electrolyte affects the adsorption of EPS and Cu(II) ions, with EPS showing higher adsorption on kaolinite in CaCl2 electrolyte. The experimental results indicate that in a mixed system, EPS contributes to immobilizing a higher amount of Cu(II) ions on clay minerals.