期刊
BIOMEDICAL OPTICS EXPRESS
卷 12, 期 5, 页码 2575-2585出版社
OPTICAL SOC AMER
DOI: 10.1364/BOE.422993
关键词
-
资金
- 5th Generation, Inc.
- National Cancer Institute [R44CA228920]
The recent application of modern microscopy techniques to human capillary imaging holds great promise for non-invasive alternatives to conventional blood tests. Techniques such as oblique plane microscopy and scattering-contrast oblique plane microscopy have been demonstrated to effectively image blood cells in human capillaries, offering a new avenue for in vivo blood analysis.
The recent application of modern microscopy techniques to human capillary imaging promises non-invasive alternatives to conventional, phlebotomy-based clinical blood tests. For example, neutropenia screening has recently been demonstrated non-invasively by imaging nailfold capillaries, relying solely on intrinsic absorption-based contrast produced by hemoglobin within red blood cells [1,2]. Capillaroscopy holds the potential to enable safer, at-home screening for immunocompromised patients. This includes cancer patients who are at increased risk of infection Oblique plane microscopy (OPM) enables high speed, volumetric fluorescence imaging through a single-objective geometry. While these advantages have positioned OPM as a valuable tool to probe biological questions in animal models, its potential for in vivo human imaging is largely unexplored due to its typical use with exogenous fluorescent dyes. Here we introduce a scattering-contrast oblique plane microscope (sOPM) and demonstrate label-free imaging of blood cells flowing through human capillaries in vivo. The sOPM illuminates a capillary bed in the ventral tongue with an oblique light sheet, and images side-and back scattered signal from blood cells. By synchronizing the sOPM with a conventional capillaroscope, we acquire paired widefield and axial images of blood cells flowing through a capillary loop. The widefield capillaroscope image provides absorption contrast and confirms the presence of red blood cells (RBCs), while the sOPM image may aid in determining whether optical absorption gaps (OAGs) between RBCs have cellular or acellular composition. Further, we demonstrate consequential differences between fluorescence and scattering versions of OPM by imaging the same polystyrene beads sequentially with each technique. Lastly, we substantiate in vivo observations by imaging isolated red blood cells, white blood cells, and platelets in vitro using 3D agar phantoms. These results demonstrate a promising new avenue towards in vivo blood analysis. (c) 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
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