期刊
NATURE COMMUNICATIONS
卷 12, 期 1, 页码 -出版社
NATURE RESEARCH
DOI: 10.1038/s41467-021-22624-z
关键词
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资金
- Harry Bubb through Cure Alzheimer's Fund [CAF-50997]
- Alzheimer's Association [NIRG-15-363477, AARF-16-440760, NIRG-394284]
- Larry Hillblom Foundation [2013-A-016-FEL, 2016-A-016-FEL]
- National Institute of Health (NIH) NIH/NIA/NINDS [AG027544, AG00538, AG54884, OD010420, U54 AG054349, AG049562, NS083801, AG056768]
- BrightFocus Foundation [A2015535S]
- Beatriz Galindo program [BAGAL18/00052]
- Institute of Health Carlos III (ISCiii) [PI18/01557]
- FEDER funds from European Union
- American federation of aging research-AFAR young investigator award
- UC Irvine startup funds
- UCI MIND pilot project
- NIH/NIA [P50 AG16573]
- Chao Family Comprehensive Cancer Center Support from the National Cancer Institute [P30CA062203]
- Minister of Science and Innovation grant [PID2019-108911RA-100]
The researchers created a mouse model that expresses wildtype human A β, demonstrating effects on cognition, synaptic plasticity, brain changes, inflammation, PAS granules, and gene expression. Furthermore, excision of exon 14 through Cre-mediated methods reduced A β expression and rescued cognitive impairments.
The majority of Alzheimer's disease (AD) cases are late-onset and occur sporadically, however most mouse models of the disease harbor pathogenic mutations, rendering them better representations of familial autosomal-dominant forms of the disease. Here, we generated knock-in mice that express wildtype human A beta under control of the mouse App locus. Remarkably, changing 3 amino acids in the mouse A beta sequence to its wild-type human counterpart leads to age-dependent impairments in cognition and synaptic plasticity, brain volumetric changes, inflammatory alterations, the appearance of Periodic Acid-Schiff (PAS) granules and changes in gene expression. In addition, when exon 14 encoding the A beta sequence was flanked by loxP sites we show that Cre-mediated excision of exon 14 ablates hA beta expression, rescues cognition and reduces the formation of PAS granules. Most instances of Alzheimer's disease (AD) are sporadic or not associated with a particular mutation. Here, the authors develop knock-in mice that express wildtype human A beta under control of the mouse App locus, which may have potential for modelling some aspects of sporadic late onset AD.
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