期刊
NATURE COMMUNICATIONS
卷 12, 期 1, 页码 -出版社
NATURE PORTFOLIO
DOI: 10.1038/s41467-021-22779-9
关键词
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资金
- Italian Ministry of Education, University and Research (MIUR) [CTN01_E48C13000140008]
- POR-FESR 2014-2020 Regione Emilia-Romagna [E8IJ10000120007, E92I16000220005]
- Lombardia e Ricerca Award
- Debra Alto Adige
- FAR 2019 [E54I19002000001]
The study demonstrates that single-cell transcriptome analysis of primary human epidermal cultures can identify different keratinocyte clonal types and reveals a hierarchy of stem cells generating progenitors and terminally differentiated cells. Holoclone-forming cells exhibit stem cell hallmarks, and FOXM1 is identified as a YAP-dependent key regulator of epidermal stem cells.
Autologous epidermal cultures restore a functional epidermis on burned patients. Transgenic epidermal grafts do so also in genetic skin diseases such as Junctional Epidermolysis Bullosa. Clinical success strictly requires an adequate number of epidermal stem cells, detected as holoclone-forming cells, which can be only partially distinguished from the other clonogenic keratinocytes and cannot be prospectively isolated. Here we report that single-cell transcriptome analysis of primary human epidermal cultures identifies categories of genes clearly distinguishing the different keratinocyte clonal types, which are hierarchically organized along a continuous, mainly linear trajectory showing that stem cells sequentially generate progenitors producing terminally differentiated cells. Holoclone-forming cells display stem cell hallmarks as genes regulating DNA repair, chromosome segregation, spindle organization and telomerase activity. Finally, we identify FOXM1 as a YAP-dependent key regulator of epidermal stem cells. These findings improve criteria for measuring stem cells in epidermal cultures, which is an essential feature of the graft. Epidermal cultures can treat skin diseases, such as Junctional Epidermolysis Bullosa, but the signature of stem cells is unclear. By single cell RNAseq analyses on human keratinocytes, the authors identify the molecular profile of holoclones and the role of FOXM1 in regulating the proliferative potential of epidermal stem cells.
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