期刊
NATURE COMMUNICATIONS
卷 12, 期 1, 页码 -出版社
NATURE RESEARCH
DOI: 10.1038/s41467-021-23197-7
关键词
-
资金
- National Key Research & Development Program of China [2019YFA0110403, 2019YFA0802000, 2018YFA0108100, 2018YFA0107900, 2019YFA0802803, 2020YFA0803202]
- National Science Foundation of China [8208810001, 31730112, 31625019, 91849202, 31922032, 81872241, 31900625, 32050087, 32070727, 31801215]
- Strategic Priority Research Program of the Chinese Academy of Sciences (CAS) [XDA16010507, XDB19000000]
- Key Project of Frontier Sciences of CAS [QYZDB-SSW-SMC003]
- Shanghai Science and Technology Commission [19JC1415700, 19YF1455300, 19ZR1479800, 20QC1401000, 18YF1427600]
- Collaborative Innovation Program of Shanghai Municipal Health Commission [2020CXJQ01]
- Pearl River Talent Recruitment Program of Guangdong Province [2017ZT07S347]
- Young Elite Scientists Sponsorship Program Grant of CAS
- Youth Innovation Promotion Association Grant
- Shanghai Yangfan Project
- Shanghai Rising Star Project
- China Postdoctoral Science Foundation
- China Postdoctoral Innovative Talent Support Program
- Royal Society-Newton Advanced Fellowship
- AstraZeneca
- Sanofi-SIBS Fellowship
- Boehringer-Ingelheim
- XPLORER PRIZE
A dual recombinase-mediated genetic system was used to trace cavity macrophages in vivo, revealing their accumulation on the surface of visceral organs during lung and liver injury without penetration into the parenchyma. Additional data suggest that these macrophages do not contribute to tissue repair and regeneration.
During injury, monocytes are recruited from the circulation to inflamed tissues and differentiate locally into mature macrophages, with prior reports showing that cavity macrophages of the peritoneum and pericardium invade deeply into the respective organs to promote repair. Here we report a dual recombinase-mediated genetic system designed to trace cavity macrophages in vivo by intersectional detection of two characteristic markers. Lineage tracing with this method shows accumulation of cavity macrophages during lung and liver injury on the surface of visceral organs without penetration into the parenchyma. Additional data suggest that these peritoneal or pleural cavity macrophages do not contribute to tissue repair and regeneration. Our in vivo genetic targeting approach thus provides a reliable method to identify and characterize cavity macrophages during their development and in tissue repair and regeneration, and distinguishes these cells from other lineages. Body cavity macrophages reside on the serous surfaces of organs and believed to participate in organ repair following injury. Here the authors show with a fate-mapping reporter system that these cells, although accumulate at the surfaces of injured liver or lung, don't penetrate deeply into the tissue.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据