期刊
NATURE COMMUNICATIONS
卷 12, 期 1, 页码 -出版社
NATURE RESEARCH
DOI: 10.1038/s41467-021-22110-6
关键词
-
资金
- Academy of Medical Sciences [SBF0031030]
- ERC [StG 852915-CRYTOCOP]
- BBSRC [BB/T002670/1]
- UK Medical Research Council [MRC_UP_1201/10]
- Wellcome Trust [202679/Z/16/Z, 206166/Z/17/Z, 102535/Z/13/Z, 109161/Z/15/A]
- Cryo-EM Service Platform at EMBL Heidelberg
- Wellcome Trust [109161/Z/15/A, 102535/Z/13/Z] Funding Source: Wellcome Trust
- BBSRC [BB/T002670/1] Funding Source: UKRI
COPII mediates the trafficking of thousands of cargoes from the Endoplasmic Reticulum to the Golgi. The complete COPII coat, consisting of two layers, plays important roles in coat organization and membrane curvature, with interactions between the layers contributing to these processes. Understanding these interactions provides insights into the regulatory mechanisms of COPII trafficking and secretion.
COPII mediates Endoplasmic Reticulum to Golgi trafficking of thousands of cargoes. Five essential proteins assemble into a two-layer architecture, with the inner layer thought to regulate coat assembly and cargo recruitment, and the outer coat forming cages assumed to scaffold membrane curvature. Here we visualise the complete, membrane-assembled COPII coat by cryo-electron tomography and subtomogram averaging, revealing the full network of interactions within and between coat layers. We demonstrate the physiological importance of these interactions using genetic and biochemical approaches. Mutagenesis reveals that the inner coat alone can provide membrane remodelling function, with organisational input from the outer coat. These functional roles for the inner and outer coats significantly move away from the current paradigm, which posits membrane curvature derives primarily from the outer coat. We suggest these interactions collectively contribute to coat organisation and membrane curvature, providing a structural framework to understand regulatory mechanisms of COPII trafficking and secretion. Cytosolic coat proteins capture secretory cargo and sculpt membrane carriers for intracellular transport, such as COPII which mediates Endoplasmic Reticulum to Golgi trafficking of thousands of cargoes. Here authors visualise the complete, membrane-assembled COPII coat by cryo-electron tomography and subtomogram averaging, revealing the full network of interactions within and between coat layers.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据