期刊
ONCOLOGY LETTERS
卷 22, 期 1, 页码 -出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/ol.2021.12800
关键词
cervical cancer; microRNA-186-3p; minichromosome maintenance complex component 2; proliferation; apoptosis; cell cycle
类别
资金
- Zhangjiakou City's 2020 Municipal Science and Technology Plan Self-Financing Project [2021053D]
The study discovered that the downregulation of miR-186-3p in cervical cancer tissues negatively regulates MCM2, leading to enhanced cell proliferation and inhibition of apoptosis. This suggests that MCM2 and its negative regulator miR-186-3p play a role in regulating cervical cancer progression.
The present study examined the effect of microRNA (miRNA/miR)-186-3p and its target gene, minichromosome maintenance complex component 2 (MCM2), on cervical cancer. Cervical cancer tissues and corresponding normal tissues were collected from 48 patients and bioinformatics analysis was performed to identify the differentially expressed genes in cervical cancer. TargetScan and TarBase were used to identify miRNAs, and reverse transcription-quantitative PCR was conducted to detect and evaluate mRNA expression levels. Additionally, MTT and 5-bromo-2-deoxyuridine assays were performed to examine cell proliferation. Cell adhesion, cell cycle distribution and apoptosis were assessed using cell adhesion, flow cytometry and caspase-3/7 activity assays, respectively. The results revealed that miR-186-3p expression was downregulated in cervical cancer tissues and cells, and it negatively regulated MCM2 expression by directly targeting its 3 ' untranslated region in cervical cancer. Furthermore, MCM2 facilitated cell proliferation and inhibited cell apoptosis, which were reversed by upregulation of miR-186-3p expression. Collectively, the present study suggested that MCM2 and its negative regulator, miR-186-3p, regulate cervical cancer progression.
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