4.5 Review

The Macrobrachium rosenbergii nodavirus: a detailed review of structure, infectivity, host immunity, diagnosis and prevention

期刊

REVIEWS IN AQUACULTURE
卷 13, 期 4, 页码 2117-2141

出版社

WILEY
DOI: 10.1111/raq.12562

关键词

aquaculture; giant freshwater prawn; Macrobrachium rosenbergii nodavirus; mechanism of infection; viral structure; white tail disease

资金

  1. Tropical Medicines and Biology Multidisciplinary Research Platform, Monash University Malaysia
  2. Fundamental Research Grant Scheme, Ministry of Higher Education, Malaysia [FRGS/1/2019/STG04/MUSM/03/2]

向作者/读者索取更多资源

The review discusses key aspects of Macrobrachium rosenbergii nodavirus, including its structure, transmission and infection mechanisms, and common strategies for detection and prevention, highlighting the importance of understanding the virus for effective outbreak control.
The Macrobrachium rosenbergii nodavirus causes white tail disease, which primarily infects giant freshwater prawns, Macrobrachium rosenbergii. The infection leads to almost 100% mortality in post-larvae, causing significant economic losses in aquaculture farms. To develop effective measures against outbreaks, a good understanding of the virus is essential. In this review, we discuss key aspects of the Macrobrachium rosenbergii nodavirus including its structure, mechanisms of transmission and infection and common strategies for detection and prevention of outbreaks. Structurally, cryogenic electron microscopy revealed that the nodavirus has a T = 3 icosahedral structure with dimeric blade-like spikes on its surface. Homology modelling comparing wild-type and enzymatically cleaved Macrobrachium rosenbergii nodavirus-like particles revealed the significance of these spikes or protruding domains for binding. In vitro and in vivo studies have identified key aspects of Macrobrachium rosenbergii nodavirus infectivity, including (i) the viral binding targets such as transglutaminase and caveolin-1, (ii) utilisation of B2-like proteins in promoting infectivity and intracellular migration, (iii) replication mechanisms and (iv) co-infection with the extra small virus. Though susceptible at a post-larvae stage, adult Macrobrachium rosenbergii is immune to Macrobrachium rosenbergii nodavirus infection. During outbreaks, polymerase chain reaction and in situ hybridisation-based detection techniques are commonly used to identify infected populations. Currently, the most useful strategies for an outbreak are physical biosecurity measures and prophylaxis such as vaccination and immunostimulants. Finally, critical gaps in research include development of immortalised shrimp cell models, elucidation of time-resolved protein changes post-infection and development of therapies to treat infections to mitigate economic losses during outbreaks.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.5
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据