期刊
EXPERIMENTAL AND THERAPEUTIC MEDICINE
卷 21, 期 6, 页码 -出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/etm.2021.9995
关键词
alliin; adipogenesis; metabolic disease; peroxisome proliferation-activity receptor γ CCAAT; enhancer-binding protein β Akt
资金
- Japan Society for the Promotion of Science (JSPS
- KAKENHI) [JP19K10192]
The study found that alliin inhibits adipocyte differentiation by reducing lipid accumulation and downregulating expression levels of multiple adipogenic differentiation-related genes. It also suppressed PKB/Akt and PI3K expression, indicating its potential therapeutic effect on metabolic diseases.
Obesity is currently an important health problem and is associated with an increased likelihood of various diseases. The efficacies of various natural treatments have been assessed for their utility in treating obesity. Alliin (S-allyl-L-cysteine sulfoxides) is considered the major component of garlic and has a wide range of natural antioxidant properties. However, the direct effects of alliin on obesity have not been well clarified. The present study investigated the effects and possible mechanisms of alliin on adipocyte differentiation. The 3T3-L1 cells were treated with alliin (0-40 mu g/ml) during adipogenic differentiation. The effect of alliin on lipid accumulation was evaluated by Oil red O staining. Reverse transcription-quantitative PCR was performed to investigate the expression levels of adipogenic differentiation-related genes. The accumulation of lipid droplets was markedly inhibited following alliin treatment. The expression levels of multiple adipogenic transcription markers, such as CCAAT/enhancer-binding protein (C/EBP) beta, C/EBP alpha and peroxisome proliferation-activity receptor gamma, were markedly decreased following treatment with alliin during adipogenic differentiation. Expression levels of several adipocyte-related genes were subsequently suppressed. Additionally, alliin suppressed PKB/Akt and PI3K expression. These results suggested that alliin exhibits anti-adipogenic activity by downregulating major adipogenic differentiation-related genes and Akt/PI3K expression. Alliin may have a potential therapeutic effect on metabolic disease.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据