期刊
CELL DEATH & DISEASE
卷 12, 期 4, 页码 -出版社
SPRINGERNATURE
DOI: 10.1038/s41419-021-03584-0
关键词
-
类别
资金
- National Natural Science Foundation of China [81870589]
- Hunan Province Natural Science Foundation of China [2017JJ2367]
- Project of Scientific Research Plan of Hunan Health Committee [B2017030, B2019135]
The study identified human PER3 as a critical positive regulator of human adipose tissue-derived stromal cells adipogenesis, enhancing adipogenesis by repressing the Notch1 signaling pathway and interacting with hHSP90AA1.
The period circadian regulator 3 (PER3) has been reported to play a negative role in human immortalized bone marrow-derived Scp-1 cells (iBMSCs) and patient adipose-derived stromal cells (PASCs) or a negative/positive role in mice adipogenesis. However, human PER3 (hPER3) was identified as a positive regulator of human adipose tissue-derived stromal cells (hADSCs) adipogenesis in this study. Silencing or overexpression of hPER3 in hADSCs inhibited and promoted adipogenesis in vitro. In vivo, the overexpression of hPER3 increased high-fat diet-induced inguinal white adipose tissue (iWAT) and epididymal white adipose tissue (eWAT) forms, increasing systemic glucose intolerance and insulin resistance. Molecularly, hPER3 does not interact with hPPAR gamma, but represses Notch1 signaling pathway to enhance adipogenesis by interacting with hHSP90AA1, which is able to combine with the promoter of hNotch1 and inactivate its expression. Thus, our study revealed hPER3 as a critical positive regulator of hADSCs adipogenesis, which was different from the other types of cells, providing a critical role of it in treating obesity.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据