期刊
FRONTIERS IN CELLULAR NEUROSCIENCE
卷 15, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fncel.2021.639754
关键词
olfactory bulb; astrocytes; norepinephrine receptors; calcium signaling; store-operated calcium entry
资金
- Deutsche Forschungsgemeinschaft [LO 779/11-1, SFB 1328 TP-A07]
The study shows that norepinephrine induces a cytosolic calcium rise in olfactory bulb astrocytes, which is independent of neuronal activity and primarily mediated by PLC/IP3-dependent internal calcium release. Additionally, store-operated calcium entry (SOCE) plays a role in the late phase of the response.
It is well-established that astrocytes respond to norepinephrine with cytosolic calcium rises in various brain areas, such as hippocampus or neocortex. However, less is known about the effect of norepinephrine on olfactory bulb astrocytes. In the present study, we used confocal calcium imaging and immunohistochemistry in mouse brain slices of the olfactory bulb, a brain region with a dense innervation of noradrenergic fibers, to investigate the calcium signaling evoked by norepinephrine in astrocytes. Our results show that application of norepinephrine leads to a cytosolic calcium rise in astrocytes which is independent of neuronal activity and mainly mediated by PLC/IP3-dependent internal calcium release. In addition, store-operated calcium entry (SOCE) contributes to the late phase of the response. Antagonists of both alpha 1- and alpha 2-adrenergic receptors, but not beta-receptors, largely reduce the adrenergic calcium response, indicating that both alpha-receptor subtypes mediate norepinephrine-induced calcium transients in olfactory bulb astrocytes, whereas beta-receptors do not contribute to the calcium transients.
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