A new genetic approach to distinguish strains of Anaplasma phagocytophilum that appear not to cause human disease

Genetic diversity of Anaplasma phagocytophilum was assessed in specimens from 16 infected patients and 16 infected Ixodes scapularis ticks. A region immediately downstream of the 16S rRNA gene, which included the gene encoding SdhC, was sequenced. For the A. phagocytophilum strains from patients no sequence differences were detected in this region. In contrast, significantly fewer ticks had a sequence encoding SdhC that was identical to that of the human strains (11/16 vs. 16/16, p = 0.04). This variation is consistent with the premise that not all A. phagocytophilum strains present in nature are able to cause clinical illness in humans. A strain referred to as A. phagocytophilumVariant-1 that is regarded as non-pathogenic for humans was previously described using a different typing method. Data from the current study suggest that both typing methods are identifying the same non-pathogenic strains.

Automated summary

The genetic diversity of Anaplasma phagocytophilum was examined in samples from infected patients and infected Ixodes scapularis ticks. While human strains showed no sequence differences, significantly fewer ticks had a sequence encoding SdhC that matched the human strains. This suggests that not all strains of A. phagocytophilum in nature can cause clinical illness in humans.