期刊
JOURNAL OF NATURAL FIBERS
卷 19, 期 13, 页码 5974-5990出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/15440478.2021.1902902
关键词
Flax; microRNAs; stem; target gene; fiber; qRT-pcr
资金
- National Natural Science Funds of China [31801411]
- Doctoral Research Fund of Guizhou University of Traditional Chinese Medicine [[2020]56]
This study revealed significant differences in miRNA expression levels during flax stem fiber development, with certain miRNAs potentially playing key roles in the plant hormone signal transduction pathway. The accuracy and reliability of these results were confirmed through qRT-PCR analysis, providing a foundation for future studies on the regulatory mechanisms of miRNAs in flax stem development.
Flax fiber was one of the earliest natural fibers utilized by humans. Current research on flax fiber development is mainly focused on functions of key genes, with few reports investigating regulation of microRNA expression. Here, miRNA-seq was employed to explore miRNA expression profiles of middle stem tissues of high-fiber flax variety Agatha-MB and low-fiber flax variety White Flower during the rapid growth period (RGP) and mature green stage (MGS) to reveal miRNAs related to flax stem fiber development. The results revealed that 24 and 26 known miRNAs were identified that exhibited significant expression differences during MGSAgatha-MB-vs-RGPAgatha-MB and MGSWF-vs-RGPWF, respectively. Forty-one miRNA-target pairs exhibited inverse expression pattern changes as compared to our previously reported transcriptome profiling results. Pathway enrichment analysis of miRNA target genes showed that the plant hormone signal transduction pathway yielded the highest number of miRNA target genes in both varieties between the two developmental stages. To confirm the accuracy and reliability of these miRNA-seq results, qRT-PCR analysis of expression changes of six selected Lus-miRNA demonstrated similar trends as observed using miRNA-seq. These findings will enhance our understanding of miRNA regulatory mechanisms in flax stem development and will provide a foundation for future functional studies of these key miRNAs.
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