期刊
VIRUSES-BASEL
卷 13, 期 3, 页码 -出版社
MDPI
DOI: 10.3390/v13030477
关键词
Aeromonas hydrophila; Aeromonas phage; Ahp2; myophage
类别
资金
- Tzu Chi University [TCIRP 98003-01]
- Ministry of Sciences and Technology of Taiwan [MOST 107-2635-B-320-003]
Ahp2 is a lytic phage that infects and lyses A. hydrophila, showing potential as a candidate for therapeutic phage cocktails. The genome of Ahp2 is closely related to other Aeromonas phages, and its tail module likely determines its host range.
Aeromonas hydrophila is an opportunistic pathogen that infects fish, amphibians, mammals, and humans. This study isolated a myophage, vB_AhyM_Ahp2 (Ahp2), that lytically infects A. hydrophila. We observed that 96% of the Ahp2 particles adsorbed to A. hydrophila within 18 min. Ahp2 also showed a latent period of 15 min with a burst size of 142 PFU/cell. This phage has a linear double-stranded DNA genome of 47,331 bp with a GC content of 57%. At least 20 Ahp2 proteins were detected by SDS-polyacrylamide gel electrophoresis; among them, a 40-kDa protein was predicted as the major capsid protein. Sequence analysis showed that Ahp2 has a genome organization closely related to a group of Aeromonas phages (13AhydR10RR, 14AhydR10RR, 85AhydR10RR, phage 3, 32 Asp37, 59.1), which infect Aeromonas hydrophila and Aeromonas salmonicida. The tail module encompassing ORF27-29 in the Ahp2 genome was present in all Aeromonas phages analyzed in this study and likely determines the host range of the virus. This study found that Ahp2 completely lyses A. hydrophila AH300206 in 3.5 h at a MOI of 0.0001 and does not lysogenize its host. Altogether, these findings show that Ahp2 is a lytic Aeromonas phage and could be a candidate for therapeutic phage cocktails.
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