期刊
VIRUSES-BASEL
卷 13, 期 3, 页码 -出版社
MDPI
DOI: 10.3390/v13030469
关键词
PEDV; CD3(+) T cells; neonatal piglets; intranasal inoculation
类别
资金
- National Natural Science Foundation of China [31930109, 31772777, 32002261]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
PEDV can survive in blood-derived CD3(+) T cells for several hours and prefers CD4(+) T cells over CD8(+) T cells. Viral transmission is mediated by cell-to-cell contact between mesenteric lymph-node-derived CD3(+) T cells, but not in blood-derived CD3(+) T cells.
Porcine epidemic diarrhea virus (PEDV) can cause intestinal infection in neonatal piglets through the nasal cavity. A process in which CD3(+) T cells carry PEDV plays a key role. However, the modes through which PEDV bridles CD3(+) T cells as a vehicle for migration to the intestinal epithelium have not been clarified. In this study, we first demonstrated that PEDV could survive in blood-derived CD3(+) T cells for several hours, depending on the multiplicity of infection. In addition, PEDV preferentially survived in CD4(+) T cells over CD8(+) T cells. Moreover, viral transmission was mediated by cell-to-cell contact between mesenteric lymph-node-derived CD3(+) T cells, but did not occur in blood-derived CD3(+) T cells. Following an increase in gut-homing integrin alpha 4 beta 7, blood-derived CD3(+) T cells carrying PEDV migrated to the intestines via blood circulation and transferred the virus to intestinal epithelial cells through cell-to-cell contact in neonatal piglets. Our findings have significant implications for understanding PEDV pathogenesis in neonatal piglets, which is essential for developing innovative therapies to prevent PEDV infection.
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