期刊
ULTRASONICS SONOCHEMISTRY
卷 72, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.ultsonch.2020.105452
关键词
Methicillin-resistant Staphylococcus aureus (MRSA); Mannosylerythritol lipid-A (MEL-A); Ultrasound; Antibacterial; Antibiofilm
资金
- National Key Research and Development Program of China [2018YFC1200100]
- Public Projects of Zhejiang Province [LGF18C200003]
- Nature Science Foundation of Zhejiang Province [LR13C200002]
The synergistic treatment of ultrasound and MEL-A showed remarkable antibacterial effect against MRSA, as evidenced by enumeration of viable microbes.
Methicillin-resistant Staphylococcus aureus (MRSA) is drug-resistant and biofilm-forming pathogenic bacteria with severe morbidity and mortality, and has been continuously detected in food products in recent years. Mannosylerythritol lipids (MELs) are novel biosurfactants and perform antibacterial property against gram -positive bacteria. Ultrasound has been applied into food sterilization as non-thermal techniques and has advantage of maintaining food nutrition and flavor over heat pasteurization. In this work, the synergistic treatment of ultrasound and MEL-A was used to combat planktonic cells and biofilm of MRSA. As a result, the combined treatment has exhibited remarkable antibacterial effect proved by enumeration of viable microbes. Furthermore, flow cytometry, scanning electron microscopy and transmission electron microscopy revealed ul-trasound has enhanced the inhibitory effect of MEL-A through exacerbating cell membrane damage. On the other hand, the collaborating working modes to eradicate MRSA biofilm were disturbing cell adhesion to surface by MEL-A and destructing mature biofilm mechanically by ultrasound, reaching to over 90% of clearance rate. The findings of this study illustrated the synergistic antimicrobial mechanism of ultrasound and MEL-A treatments, and offered theoretical basis for their potential applications in food preservation.
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