期刊
TRENDS IN PARASITOLOGY
卷 37, 期 5, 页码 367-369出版社
CELL PRESS
DOI: 10.1016/j.pt.2021.03.002
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资金
- Intramural Research Program of the National Institute of Allergy and Infectious Diseases, National Institutes of Health
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2013/50724-5, 2014/50897-0]
- CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico) [465651/20143, 424729/201]
- FAPESP [2018/09948-0]
By utilizing CRISPR/Cas9 technology, researchers successfully dissected the function of 204 kinases in the life cycle of Leishmania mexicana.
Our understanding of regulatory factors in Leishmania differentiation has long been restricted by the available genetic tools, but the availability of CRISPR/Cas9 has changed the landscape forever. Recently, Baker and Catta-Preta et al. applied Cas9 editing and kinome-wide bar-seq to dissect the function of 204 kinases in the Leishmania mexicana life cycle.
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