期刊
TRANSFUSION
卷 61, 期 6, 页码 1867-1883出版社
WILEY
DOI: 10.1111/trf.16402
关键词
oxidant stress; PUFA; HUFA; pyruvate; lactate; RBCs; transfusion medicine
类别
资金
- National Heart, Lung, and Blood Institute [HHSN2682011-00001I, HHSN2682011-00002I, HHSN2682011-00003I, HHSN2682011-00004I, HHSN2682011-00005I, HHSN2682011-00006I, HHSN2682011-00007I, HHSN2682011-00008I, HHSN2682011-00009I, R01HL146442, R01HL148151, R01HL149714, R21HL150032, T32 HL007171]
- National Institute of General Medical Sciences [RM1GM131968]
- Boettcher Webb-Waring Investigator Award
The study found that fatty acid desaturases are present and active in mature RBCs, and their activity is sensitive to oxidant stress, storage duration, and alterations of the pyruvate/lactate ratio.
Background Increases in the red blood cell (RBC) degree of fatty acid desaturation are reported in response to exercise, aging, or diseases associated with systemic oxidant stress. However, no studies have focused on the presence and activity of fatty acid desaturases (FADS) in the mature RBC. Study design and methods Steady state metabolomics and isotope-labeled tracing experiments, immunofluorescence approaches, and pharmacological interventions were used to determine the degree of fatty acid unsaturation, FADS activity as a function of storage, oxidant stress, and G6PD deficiency in human and mouse RBCs. Results In 250 blood units from the REDS III RBC Omics recalled donor population, we report a storage-dependent accumulation of free mono-, poly-(PUFAs), and highly unsaturated fatty acids (HUFAs), which occur at a faster rate than saturated fatty acid accumulation. Through a combination of immunofluorescence, pharmacological inhibition, tracing experiments with stable isotope-labeled fatty acids, and oxidant challenge with hydrogen peroxide, we demonstrate the presence and redox-sensitive activity of FADS2, FADS1, and FADS5 in the mature RBC. Increases in PUFAs and HUFAs in human and mouse RBCs correlate negatively with storage hemolysis and positively with posttransfusion recovery. Inhibition of these enzymes decreases accumulation of free PUFAs and HUFAs in stored RBCs, concomitant to increases in pyruvate/lactate ratios. Alterations of this ratio in G6PD deficient patients or units supplemented with pyruvate-rich rejuvenation solutions corresponded to decreased PUFA and HUFA accumulation. Conclusion Fatty acid desaturases are present and active in mature RBCs. Their activity is sensitive to oxidant stress, storage duration, and alterations of the pyruvate/lactate ratio.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据