Effects of intravenous administration of recombinant Phα1β toxin in a mouse model of fibromyalgia

This study investigated the effects of intravenous (iv) administration of recombinant Ph alpha 1 beta toxin, pregabalin, and diclofenac by the intrathecal route using an animal model fibromyalgia (FM). The reserpine administration (0.25 mg/kg s. c) once daily for three consecutive days significantly induced hyperalgesia, immobility time, and sucrose consumption in mice on the 4th day. Reserpine caused hyperalgesia on the mechanical and thermal hyperalgesia on the 4th day was reverted by recombinant Ph alpha 1 beta (0.2 mg/kg iv) and pregabalin (1.25 mu mol/site i. t) treatments. In contrast, diclofenac (215 nmol/site i. t) was ineffective. Recombinant Ph alpha 1 beta toxin, pregabalin, and diclofenac did not affect the depressive-like behavioural effect induced by reserpine on mice during the forced swim and sucrose consumption tests. The data confirmed the analgesic effect of the recombinant Ph alpha 1 beta toxin administered intravenously in a fibromyalgia mouse model.

Automated summary

The study found that recombinant Ph alpha 1 beta toxin and pregabalin had a certain therapeutic effect on reserpine-induced mechanical and thermal hyperalgesia, but diclofenac was ineffective. These drugs did not affect the depressive-like behavioral effect induced by reserpine.